A water-soluble molecular fluorescence probe，dimethyl curcumin（ASC-J9）liposome was developed for the recognition of Fe3+，Fe2+ and Cu2+ through fluorescence quenching.The ASC-J9 liposome was prepared by thin-film dispersion，which had a uniform particle size distribution with a particle size of 145.7 nm and a polymer dispersity index（PDI）of 0.361.The optimal fluorescence conditions obtained by conditional screening were as follows：ASC-J9 liposome concentration：5.0×10-5 mol/L，balance time：5 min，temperature：25 ℃.The binding modes of ASC-J9 liposome with Fe3+，Cu2+ and Fe2+ were achieved by Jobs plot and fluorescence titration.Results showed that the binding ratios for the probe to Fe3+ and Cu2+ were both 1∶1，while that for the probe to Fe2+ was 2∶1.The fluorescence quenching type was determined by Stern-Volmer plot as static quenching，and the association constants of KFe（Ⅲ），KFe（Ⅱ）and KCu（Ⅱ）were 9.63×104 L/mol，3.65×105 L/mol and 2.32×105 L/mol，respectively.With the advantages of rapid detection and high sensitivity，this method was applied in the identification of Fe3+，Fe2+ and Cu2+ ions in water samples.The calibration curves for Fe3+，Fe2+ and Cu2+ were 5.0×10-7 -3.0×10-5 mol/L，5.0×10-7 -1.75×10-5 mol/L and 5.0×10-7 -2.5×10-5 mol/L，with their limits of detection of 6.41×10-7 mol/L，3.28×10-7 mol/L and 5.08×10-7 mol/L，respectively.