A method for the determination of 187 kinds of pesticides in dried persimmon by liquid chromatography-tandem mass spectrometry（LC-MS/MS） was established.The samples were extracted with acetonitrile containing 1% acetic acid，then purified with anhydrous magnesium sulfate，anhydrous sodium acetate，anhydrous sodium sulfate，N propyl ethylenediamine（PSA） and C18.The separation of targeted compounds was performed on a ZORBAX Eclipse Plus-C18 column by gradient elution using 95% acetonitrile aqueous solution（organic phase） and water（aqueous phase） as mobile phases，both contained 0005 mol/L ammonium formate and 001% formic acid.The compounds were detected by LC-MS/MS with positive electrospray ion source（ESI+） under dynamic multiple reaction monitoring（DMRM） mode.The matrix-matched external standard method was used for the quantitation.The callibration curves for 187 pesticides were linear in the range of 1-100 μg/kg with correlation coefficients（r2） more than 099.The limits of detection（S/N≥3） and quantitation（S/N≥10） for the analytes were 1 μg/kg and 10 μg/kg，respectively.The recoveries were in the range of 702%-118% with relative standard deviations（RSDs，n=6） of 20%-20%.The method is suitable for the high throughput detection on pesticide multi residues in dried persimmon with its advantages of accuracy，sensitivity and rapidness.