An ultra performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS） method was developed for the simultaneous analysis of 10 antiviral drugs,eg.valaciclovir,abacavir,entecavir,zidovudine,nevirapine,ganciclovir,telbivudine,lamivudine,acyclovir and amantadine in surface water.The aqueous samples were concentrated and purified on an ISOLUTE ENV+ cartridge,and then separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution with 01% formic acid-acetonitrile as mobile phase.The detection of antiviral drugs was performed by tandem mass spectrometry in electrospray ionization under multiple reactions monitoring（MRM） mode.The calibration curves for target compounds were linear over the range of 05-200 ng/L with correlation coefficients（r2） larger than 0993.The average recoveries for antiviral drugs in surface water at three spiked concentration levels ranged from 700% to 1040%,with the limits of quantitation（LOQ） of 02-50 ng/L and the relative standard deviations（RSDs） less than 15%.This method could be applied in the analysis of antiviral drugs in aqueous samples.