A comprehensive analytical method was developed for the simultaneous determination of nine uric acid regulation drugs illegally added in Chinese traditional patent medicine and health-care food by liquid chromatography-tandem mass spectrometry（LC-MS/MS）.The sample was dipersed with water，and the analytes were ultrasonically extracted using vortex mixer with acetonitrile.The chromatographic separation was performed on a Poroshell 120 Bonus-RP chromatographic column（100 mm×2.1 mm，2.7 μm），with 0.1%（by volume）acetic acid aqueous solution containing 5 mmol/L ammonium formate and acetonitrile as mobile phases by gradient elution.The qualitative and quantitative analyses were performed by electrospray ionization mass spectrometry in positive mode under multiple reaction monitoring（MRM）mode.Results showed that the calibration curves for nine drugs were linear in the corresponding concentration ranges with their correlation coefficients larger than 0.99.The average recoveries for 9 drugs at three spiked levels ranged from 79.2% to 108%，with relative standard deviations（RSDs）of 1.2%-12%.The limits of detection（LOD）and quantitation（LOQ）were in the ranges of 0.03-0.6 mg/kg and 0.1-2.0 mg/kg，respectively.The established method is simple，rapid，sensitive，accurate and reliable，and is suitable for the determination of nine uric acid regulation drugs illegally added in Chinese traditional patent medicine and health-care food.