A novel flow injection-chemiluminescence(FI-CL) method was established for the determination of praziquantel based on the strong inhibition of praziquantel on Luminol-NaIO4 chemiluminescence system.Under the optimal experimental conditions,there was a good linear relationship between relative intensity of chemiluminescence and praziquantel in the concentration range of 40×10-8-10×10-6 mol/L,with a limit of detection(LOD) of 99×10-9 mol/L.The relative standard deviation(RSD,n=11) was 14% for the determination of 20×10-7 mol/L praziquantel.The proposed method was used for the detection of praziquantel in the spiked pig feed sample with recoveries of 985%-100%.The method was successfully applied in the determination of praziquantel in pig feed with satisfactory results.