In this paper,a quantitative detection system of cardiac biomarkers was proposed and demonstrated,which could be used in the detection of matrix metallopeptidase 9（MMP9),growth stimulation expressed gene 2（ST2) and pentraxin 3（PTX3).The reaction product of cardiac biomarkers was captured with smartphone camera.The photo was sent to the server side for analysis via the smartphone app.The detection results were displayed on the smartphone screen after the captured photo was analyzed by the server side.Results showed that the calibration curves for of three cardiac biomarkers based on the blue channel agreed well with the four parameters fitted model,and their correlation coefficients（r2 ) were 0999 7,0999 8 and 0999 8 respectively.41,43 and 40 serum samples were detected with MMP9,ST2 and PTX3,respectively.The comparisons between three detection results by the proposed method with those by commercial reader showed that the overall agreements reached up to 9512%,9767% and 9500%,respectively.The proposed method is suitable for the point of care detection and long term monitoring of cardiac biomarkers.