A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was developed for the rapid determination of six anesthetics,eg.procaine,lidocaine,bupivacaine,tetracaine,tricaine methanesulfonate(MS-222) and benzocaine in aquatic products.The samples were extracted with water and acetonitrile containing 1.0% formic acid,and then cleaned up with a PRiME HLB pass through solid phase extraction column.The targets were separated on a ZORBAX Eclipse XDB-C18 column(3.0 mm×150 mm，3.5 μm) with acetonitrile-0.1% formic acid as mobile phase by gradient elution.The analytes were determined in electrospray positive ionization(ESI+) mode under multiple reaction monitoring(MRM).Results showed that there were good linear relationships for the targets in their respective concentration ranges with correlation coefficients(r2) of 0.993 8-0.998 7.The limits of detection(LOD) and limits of quantitation(LOQ) were in the ranges of 1.5-6.0 μg/kg and 5.0-20 μg/kg,respectively.Average spiked recoveries for six anesthetics were in the range of 76.8%-110% with relative standard deviations of 4.2%-11%.The proposed method is simple,quick,accurate and sensitive,and is suitable for the determination of six anesthetics in aquatic products.