A method was established for the simultaneous determination of carnosine and anserine in animal derived food extracts by ultrahigh performance liquid chromatography-electrospray ionization triple quadrupole tandem mass spectrometry.The separation on the analytes was performed by an ultrahigh performance liquid separation system with an Inertsil Amide HP(2.1 mm×100 mm,3 μm) column using 0.1% acetic acid and acetonitrile as mobile phases by gradient elution,and the external standard method of peak area was used for quantitation.There existed good linear relationships for the peak areas of carnosine and anserine in the concentration range of 1.95-500 μg/L,with their correlation coefficients(r) larger than 0998.The detection limits for carnosine and anserine were 0.12 μg/L and 0.47 μg/L,respectively.The low concentration oligopeptide raw material was used as the substrate,and the quality control of 2,20,and 200 μg/L was spiked.The recoveries for carnosine and anserine at three spiked levels of 2,20 and 200 μg/L were in the ranges of 993%-109% and 100%-113%,with their relative standard deviations(RSD,n=6) of 0.98%-1.1% and 1.0%-1.1%,respectively.With the advantages of simple pretreatment,rapidness,good reproducibility and high precision,the method is applicable for the simultaneous detection of carnosine and anserine in animal derived food extracts.