An ultra high performance liquid chromatography-tandem mass spectrometric(UHPLC-MS/MS) method was established for the determination of spinetoram residues,XDE-175-J and XDE-175-L in Radix Gotonopsic Tongshen Otiv.The samples were exacted with acetonitrile,and purified with primary secondary amine and graphitized carbon black.The target compounds were quantified by the external standard method.There were good linearities for the peak areas of XDE-175-J and XDE-175-L in the concentration ranges of 0075-75 μg/L and 0025-25 μg/L,respectively.The average recoveries for XDE-175-J and XDE-175-L at the spiked levels of 0375,375,75 μg/kg and 0125,125,25 μg/kg were in the ranges of 884%-1135% and 844%-995%,with relative standard deviations(RSDs) of 20%-42% and 25%-49%,respectively.The limits of quantitation(LOQs) for XDE-175-J and XDE-175-L were 0375 μg/kg and 0125 μg/kg,respectively.With the advantages of simplicity,rapidness and convenience,the method could meet the requirements for pesticide residues analysis,and be applied in the rapid detection of spinetoram residues in Radix Gotonopsic Tongshen Otiv.