Based on C8 modified macroporous silica,a coated cellulose tri(4 methylbenzoate)(CTMB) chiral stationary phases(CSPs) were prepared.Influences of coating solvent and additive on the enantioselectivity of CSP were investigated.Several CSPs were prepared by coating CTMB in chloroform and dichloromethane,with N,N’ dimethylformamide(DMF),phenol,nitrobenzene,methyl benzoate and acetophenone as additives,respectively.The enantioselectivities of CSPs were evaluated by using 7 structurally different enantiomers.It was found that column efficiency,retention ability and enantioselectivity of CSPs were affected by the type and amount of additive.The addition of the same amount of methyl benzoate with CTMB would provide the CSPs with better properties,which exhibit a good enantioseparation ability for racemic simendan intermediate and ketorolac,and possess an application potential in industrial preparation and separation.