A liquid chromatography-tandem mass spectrometric（LC-MS/MS） method was developed for the quantitative determination of aflatoxin B1（AFB1）,aflatoxin M1（AFM1）,T-2 toxin（T-2）,HT-2 toxin（HT-2）,deoxynivalenol（DON）,ochratoxin A （OTA） and zearalenone（ZEN） in shrimp feed.Samples were extracted with 15 mL acetonitrile-water（4∶1,by volume）,and degreased with 10 mL acetonitrile saturated hexane.The extract was cleaned up with a MycoSpinTM400 multitoxin LC-MS/MS clean-up column.The target compounds were separated on an Hypersil Gold（150 mm×2.1 mm,5 μm）chromatographic column by gradient elution with methanol-water containing 0.03%ammonia as mobile phase,and analyzed simultaneously by electron spray ionization mass spectrometry in positive or negative ion mode,and quantified by the matrix-matched external standard method.Under the optimized conditions,there were good linearities for the analytes in corresponding concentration range with their correlation coefficients（r2） more than 0.991.The limits of detection（LOD）ranged from 1.83 μg/kg to 12.63 μg/kg.Average recoveries for the target mycotoxins at high,medium and low spiked levels ranged from 87.5%to 116%with relative standard deviations of 2.4%-18%.The developed method could provide a reference for the simultaneous determination of mycotoxins in aquatic feeds.