In this paper,the flowerlike MoS2/GO/o-MWNTs composites were synthesized by using Na2MoO4?2H2O as molybdenum source,thiourea as sulfur source and reducing agent,and mixed oxidized multi-walled carbon nanotubes(o-MWNTs) and graphene oxide(GO) as raw material,respectively.Then,the gold nanoparticles were deposited on the surface of MoS2/GO/o-MWNTs by in situ chemical reduction method.The characterization of this nanocomposites was performed by field emission scanning electron microscopy(FESEM),transmission electron microscopy(TEM),X-ray diffraction(XRD) and X-ray photoelectron spectroscopy(XPS).Results demonstrated that the MoS2/GO/o-MWNTs composites were synthesized successfully via the hydrothermal method,and gold nanoparticles were modified on the flower sheets.The nanocomposites were modified on the glassy carbon electrode(GCE) to investigate the electrochemical behavior for H2O2.The results showed that the electrode exhibited a strong catalytic activity toward H2O2，and there was a linear relationship between the electrode and H2O2 in the concentration range of 12.0×10-9-31.0×10-6 mol/L with a sensitivity of 56.6 μA/(mmol?L-1) and a detection limit of 12.0×10-9 mol/L.