A method was established for the determination of 11 marine biotoxins in seafood by high performance liquid chromatography-quadrupole electrostatic field orbitrap high resolution mass spectrometry(HPLC-Q-Orbitrap HRMS).It was a one stop extraction and purification system for hydrophilic and lipophilic marine biotoxins.Samples were firstly treated by sequential extraction procedure,then cleaned up by disperse solid phase extraction(dSPE) combined with carrier assisted liquid-liquid extraction(SLLE).Under the optimal conditions,the calibration curves for 11 marine biotoxins were linear in the certain concentration ranges,with correlation coefficients greater than 0.99.The limits of detection(LOD) and limits of quantitation(LOQ) were in the ranges of 1-10 μg/kg and 2-20 μg/kg,respectively.The spiked recoveries ranged from 55.6% to 122% with relative standard deviations(RSD) of 5.4%-16%.The method was simple,efficient and operable,and could solve the problem of high throughput detection of hydrophilic and lipophilic marine biotoxins in seafood and meet the requirements for rapid screening.