A QuEChERS/high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method was developed for the determination of 61 kinds of hormone residues in soil.Samples were extracted with acetonitrile,and then purified using QuEChERS method.Analysis on the targets was performed by electrospray ionization mass spectrometry in positive or negative mode,multiple reaction monitoring(MRM) mode was used for collection,and matrix matched standard curve method was selected for quantitation.Results showed that 61 analytes showed good linearities in their respective ranges,with correlation coefficients(r2) between 0.991 8 and 0.999 8.The limits of detection(LOD,S/N≥3) were 0.01-2.3 μg/kg while the limits of quantitation(LOQ,S/N≥10) were 0.03-7.5 μg/kg.Recoveries for the target analytes at spiked levels of 10,50 and 200 μg/kg ranged from 62.6% to 102%,with relative standard deviations(n=6) of 1.0%-11%.The method is simple,rapid,replicable and high stable,and could meet the requirements for determination of 61 hormone residues in soil.