Vitamin D is a fat soluble vitamin essential to human health,of which 25 hydroxyvitamin D is the major circulation and storage mode in human body.Dietary intake is an important way to obtain vitamin D and 25-hydroxyvitamin D for human.The pretreatment methods for vitamin D and 25-hydroxyvitamin D in foods usually include alkaline saponification,organic solvent extraction and purification by solid phase extraction or semi preparative chromatography.Traditionally,radioimmunoassay technique and high performance liquid chromatography were used for the determination of vitamin D and 25-hydroxyvitamin D in foods.Nowadays,liquid chromatography tandem mass spectrometry with higher accuracy and precision has become one of the most important methods in determination of vitamin D and 25-hydroxyvitamin D in foods.Recently,two dimensional liquid chromatography and ultrahigh performance supercritical fluid chromatography have gradually showed a powerful potential in detection of vitamin D and 25-hydroxyvitamin D in foods for their great separation abilities.The determination techniques for vitamin D and 25-hydroxyvitamin D and their content distributions in animal and plant foods are summarized in this review,in order to provide a reference for future studies such as developing suitable determination methods for different kinds of foods,determining their contents,providing reasonable diet suggestions for residents and dietary intake assessment.