A smartphone method based on molecularly imprinted kit was developed for the determination of glucose in blood.The imprinted kit was composed of glucose molecularly imprinted paper and chromogenic agent.The glucose in blood was specifically combined with the obtained glucose imprinted paper,other impurities were eluted with chloroform.Then,chromogenic reagent was added into the surface of the test paper for coloration.UV-Vis spectra showed the imprinted paper adsorbed the glucose in blood with an adsorption time of 5 min and a maximum adsorption capacity of 0673 mg per piece.The generated color was detected by the combination of smartphone photographing with a free software GIMP 28.Results showed that there existed a good linear relationship between the chromaticity(H) and the negative logarithm of glucose concentration(pC),with a linear equation of H=421-233pC and a correlation coefficient(r) of 0993.The method was applied in the determination of glucose in rabbit blood with recoveries of 918%-108%.Compared with blood glucose meter,this method is cheaper and more convenient.