In this paper

an electrochemical immunosensor for the detection of bisphenol A(BPA) was constructed

based on the preparation of a poly-L-lysine/nano-gold modified electrode by electrodeposition

with disposable screen printed carbon electrode as the substrate.Results showed that

when the dilution factors for the antigen and the primary antibody were 1 000 times

the incubation time for the primary antibody was 60 min

and the pH value for the electrochemical detection environment was 7.4

there existed a good linear relationship between reduction peak current and BPA in the concentration range of 1-250 ng/mL

with a correlation coefficient(r2) of 0.993 and a detection limit of 0.85 ng/mL.With good specificity

reproducibility and stability

this method was applied in the detection of BPA in samples with spiked recoveries of 983%-104%

indicating that the BPA immunosensor has an application potential in the detection of bisphenol A in actual water samples.