An analytical method for the metabolomics of Lonicera japonica was established based on liquid chromatography-mass spectrometry(LC-MS).The extraction solution was optimized as a mixture solution of methanol and water(3∶1

by volume).The repeatability and precision of the method were investigated.It was found that the method could meet the requirements for metabolomics analysis with good stability.The primary and secondary metabolites in Lonicera japonica were identified by accurate mass

retention time and MS/MS data.A total of 157 metabolites were identified

including amino acids

nucleosides

fatty acids

lipids

phenolic acids

flavonoids and iridoid glycosides

etc.According to the metabolomics analysis of Lonicera japonica in different flowering stages

it was found that the secondary metabolites of phenolic acid

flavonoids and iridoid glycosides decreased significantly with the process of flowering stages.For the primary metabolites

the contents of most amino acids

nucleosides and oxidized fatty acids decreased significantly

while the contents of most Lyso PC

Lyso PE and fatty acids increased significantly.The results obtained provide theoretical guidances for study on the metabolic mechanism of medicinal components and the late planting and harvesting of Lonicera japonica.