基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定全血及尿液中的百草枯

陈超; 罗辉泰; 张秋炎; 杨运云; 向章敏; 刘舒芹

doi:doi：10.3969/j.issn.1004-4957.2020.09.010

您当前的位置：

首页 >

文章列表页 >

基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定全血及尿液中的百草枯

更新时间：2023-04-18

- 基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定全血及尿液中的百草枯
- Rapid Determination of Paraquat in Whole Blood and Urine by Matrix Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
- 分析测试学报 2020年39卷第9期页码：1126-1130
- 作者机构：
  
  广东省科学院广东省测试分析研究所(中国广州分析测试中心)广东省原位电离质谱分析工程技术研究中心
- 作者简介：
- 基金信息：
  
  广东省科学院发展专项资金项目(2020GDASYL-20200103047)
- DOI：doi：10.3969/j.issn.1004-4957.2020.09.010
  中图分类号：
- 纸质出版：2020
- 稿件说明：
移动端阅览
陈超, 罗辉泰, 张秋炎, 杨运云, 向章敏, 刘舒芹. 基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定全血及尿液中的百草枯[J]. 分析测试学报, 2020,39(9):1126-1130.

陈超, 罗辉泰, 张秋炎, 杨运云, 向章敏, 刘舒芹. Rapid Determination of Paraquat in Whole Blood and Urine by Matrix Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry[J]. 2020, 39(9): 1126-1130.
陈超, 罗辉泰, 张秋炎, 杨运云, 向章敏, 刘舒芹. 基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定全血及尿液中的百草枯[J]. 分析测试学报, 2020,39(9):1126-1130. DOI： doi：10.3969/j.issn.1004-4957.2020.09.010.

陈超, 罗辉泰, 张秋炎, 杨运云, 向章敏, 刘舒芹. Rapid Determination of Paraquat in Whole Blood and Urine by Matrix Assisted Laser Desorption Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry[J]. 2020, 39(9): 1126-1130. DOI： doi：10.3969/j.issn.1004-4957.2020.09.010.

摘要

该文建立了一种利用基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱(MALDI-FTICR-MS)快速测定全血及尿液中百草枯的分析方法。采用含1%甲酸的乙腈溶液从生物样品中提取百草枯，并与2

5-二羟基苯甲酸溶液混合后直接进样分析。结果表明，全血、尿液和水中的百草枯在10~5 000 μg/L质量浓度范围内线性良好，相关系数(r)为0.995 5~0.999 2；3种基质下的方法检出限(LOD

S/N=3)为0.6~3.0 μg/L，定量下限(LOQ，S/N=10)为2.0~10 μg/L；百草枯在全血和尿液中的平均回收率为85.2%~110%，相对标准偏差(RSD)为0.40%~7.3%。该方法具有所需样品和溶剂用量少，操作简单，分析速度快，灵敏度高，且稳定可靠等特点，可满足临床及司法鉴定相关的中毒快检需求。

Abstract

A matrix assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometric(MALDI-FTICR-MS) method was developed for rapid determination of paraquat in whole blood and urine.The sample was extracted by liquid-liquid extraction method

with acetonitrile solution containing 1% formic acid as extraction solvent.Prior to analysis

the extracted solution was mixed with 2

5-dihydroxybenzoic acid.Results showed that there were good linear relationships for paraquat in whole blood and urine samples in the mass concentration range of 10-5 000 μg/L

with correlation coefficients(r) of 0.995 5-0.999 2.The limits of detections(LODs) and limits of quantitations(LOQs) of the method were in the ranges of 0.6-3.0 μg/L and 2.0-10 μg/L

respectively.The average recoveries at spiked levels ranged from 85.2% to 110%

with relative standard deviations(RSDs) of 0.40%-7.3%.The method showed the advantages of low sample and solvent consumption

simple operation

rapid analysis and high sensitivity

and could meet the requirements for rapid detection of clinical poisoning and forensic.

关键词

Keywords

references

浏览量

325

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱法快速测定蔬菜中百草枯与敌草快

超高效液相色谱-四极杆飞行时间质谱分析尿中2-FXE及其代谢物

氮氯共掺杂碳量子点的制备及其对6种四环素类抗生素的检测

固相萃取结合液相色谱-串联质谱法同时检测人体尿液中对苯二胺类抗氧化剂及其醌类衍生物

垃圾焚烧厂周边土壤中微塑料质量丰度的Py/GC-MS和TD/GC-MS比较研究