This study aimed at the investigation of effect of Xuefu Zhuyu decotion(XFZY) on rats with traumatic brain injury(TBI) in plasma metabolomics.The plasma samples from the treatment group

the model group as well as the sham group were analyzed by gas chromatography-mass spectrometry(GC-MS).Forty three important metabolites were qualitatively and quantitatively analyzed.t test was employed for the model group and the sham group

and 13 metabolites were found with significant differences.Orthogonal partial least squares-discriminant analysis(OPLS-DA) was performed on the model group and the sham group.The obvious classification tendency was observed between the two groups.Integrating the variable importance in projection(VIP) and the results of T test

lactic acid

histidine

palmitic acid

tryptophan

linoleic acid

oleic acid

and stearic acid were selected as biomarkers.The treatment group was gavaged with XFZY

and seven biomarkers were used to observe the metabolite changes in treatment groups experiencing the treatment after 1

3

7 and 14 days

respectively.It was found that the relative concentrations of lactic acid

histidine

palmitate

linoleic acid

and stearic acid decreased gradually.The relative concentration of tryptophan decreased at first

and then increased

while the relative concentration of oleic acid showed an opposite trend.A consensus is emerging about the relative concentration of seven metabolites between the sham group and treatment group on day 14.Experimental results showed that XFZY had a good therapeutic effect on TBI.Meanwhile

those metabolites

ie lactic acid

histidine

palmitic acid

tryptophan

linoleic acid

oleic acid and stearic acid could be used as the biomarkers for monitoring the therapeutic effect and recovery of TBI.