A sensor for the detection of enzyme free glucose was fabricated with three dimensional Cu2O@Cu/TiO2 NTAs nanocomposites.Firstly

the three dimensional TiO2 nanotube arrays(TiO2 NTAs) were prepared by anodizing

and then a Cu film was electrodeposited on the surface of TiO2 NTAs by square wave pulse method.After calcination

the Cu2O@Cu/TiO2 NTAs nanocomposites were prepared.Results of X-ray diffractometer showed that Cu and Cu2O coexisted in the material.Combined with the analysis of SEM and TEM

Cu2O was uniformly grown on the outer surface of TiO2 tube wall

and the results of selected area electron diffraction displayed that the material presented a mixed crystal structure.The differential pulse voltammetry(DPV) showed that the oxidation peak of Cu2O@Cu/TiO2 NTAs was obvious at +0.56 V

indicating that the nanocomposite has strong electrocatalytic effect for glucose and could be used to detect it.There was a linear relationship of the method for glucose in the range of 0.19-35 mmol/L

with a sensitivity of 372.0 μA·L·mmol-1·cm-2 and a detection limit of 3 μmol/L.The electrochemical impedance exhibited that charge transfer resistance of Cu2O/TiO2 NTAs was 526.2 Ω

which was 292 times higher than that of Cu2O@Cu/TiO2 NTAs(merely 1.8 Ω).It displayed that Cu as the middle transition layer reduced the electronic conduction impedance.The sensor was used for the determination of glucose in serum sample.There was no significant difference between the results of the test and those of the hospital.Cu2O@Cu/TiO2 NTAs could be used as an enzyme free glucose sensor with its high sensitivity

fast response and good selectivity.