严梅敏, 李丽娜. Research on Quantitative Analysis of Crystal Violet Using a CdTe Fluorescence Probe Modified by 2-Hydroxypropyl-β-Cyclodextrin and Its Mechanism[J]. 2020, 39(2): 252-257.
严梅敏, 李丽娜. Research on Quantitative Analysis of Crystal Violet Using a CdTe Fluorescence Probe Modified by 2-Hydroxypropyl-β-Cyclodextrin and Its Mechanism[J]. 2020, 39(2): 252-257.DOI: doi:10.3969/j.issn.1004-4957.2020.02.013.
2-Hp-β-CDCdTe QDs for the detection of crystal violet(CV) was fabricated in the presence of TGA as stabilizer and 2-hydroxypropyl-β-cyclodextrin(2-Hp-β-CD) as modifier
which showed high fluorescence intensity
water solubility and light stability
as well as good inclusion ability for CV.The interaction between 2-Hp-β-CDCdTe QDs and CV was investigated by UV-Vis
fluorescence spectroscopy and TEM.The data indicated that the distance between the donor and the acceptor was reduced significantly by the action of CTAB
and the energy transfer between the QDs and CV was promoted.The fluorescence spectrum for QDs could overlap the absorption spectrum of CV effectively.Based on this
the fluorescence resonance energy transfer(FRET) system between QDs and CV was established
which could be used for the determination of CV.Results showed that in pH 8.0 Tris-HCl buffer
CV could quench the fluorescence peak of 2-Hp-β-CDCdTe QDs in the presence of CTAB
and there was a good linear relationship between the change of fluorescence intensity(ΔF) and CV concentration in the range of 1.0×10-7-1.0×10-5 mol/L(r2=0995 2).The detection limit was 1.74×10-8 mol/L
and the average recoveries were in the range of 99.0%-106%.