A method of ultra high performance liquid chromatography(UHPLC) was developed for the simultaneous determination of eight components

ie.chlorogenic acid

cryptochlorogenic acid

rutin

nicotifiorin

hederacoside C

hederacoside D

hederasaponin B and α hederin in Hedera helix L..The dry leaves passed through 40 mesh sieve were put in 80% methanol with solid-liquid ratio of 1∶100.The solution then was refluxed by water bath for 1 h at 85 ℃ to prepare the test solution.The chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus-C18(2.1 mm×100 mm

1.8 μm) column by gradient elution with acetonitrile and 0.05%phosphoric acid as mobile phases.Results indicated that the eight components in Hedera helix L.were well separated

and the relative standard deviations(RSD) of the method for precision and reproducibility were less than 3.0%.The eight components were found to be stable for 24 h in room temperature

and there existed good linear relationships for the analytes in corresponding mass concentration ranges with their correlation coefficients(r) not less than 0.999 5.The limits of detection and the limits of quantitation were in the ranges of 0.40-10.58 μg·mL-1 and 1.31-34.61 μg·mL-1

respectively.Average recoveries for the 8 components were between 97.3%and 108%with RSD(n=6) of 0.51%-3.2%.Therefore

this method could be applied in the quantitative analysis of the 8 components in Hedera helix L..