信建豪, 张翠利, 张利娟, 王欢欢, 李锟. Determination of Glucose in Blood Using Smart Phone Based on Molecularly Imprinted Kit[J]. 2019, 38(7): 849-853.DOI: doi:10.3969/j.issn.1004-4957.2019.07.014.
建立了一种基于葡萄糖印迹检测盒的智能手机检测血液中葡萄糖的方法。以葡萄糖印迹试纸与显色剂构成检测盒,血液中的葡萄糖与检测盒中的葡萄糖印迹试纸产生特异性结合后,用氯仿洗脱杂质,在试纸表面加入显色剂使之反应显色,再用智能手机拍照并结合免费软件GIMP 28检测色度。结果表明,制备的分子印迹试纸对葡萄糖的吸附时间为5 min
A smartphone method based on molecularly imprinted kit was developed for the determination of glucose in blood.The imprinted kit was composed of glucose molecularly imprinted paper and chromogenic agent.The glucose in blood was specifically combined with the obtained glucose imprinted paper
other impurities were eluted with chloroform.Then
chromogenic reagent was added into the surface of the test paper for coloration.UV-Vis spectra showed the imprinted paper adsorbed the glucose in blood with an adsorption time of 5 min and a maximum adsorption capacity of 0673 mg per piece.The generated color was detected by the combination of smartphone photographing with a free software GIMP 28.Results showed that there existed a good linear relationship between the chromaticity(H) and the negative logarithm of glucose concentration(pC)
with a linear equation of H=421-233pC and a correlation coefficient(r) of 0993.The method was applied in the determination of glucose in rabbit blood with recoveries of 918%-108%.Compared with blood glucose meter