The development course of electron microscopy(EM) and its new functions in recent years are introduced in this article.In bio nanomaterials research

EM could be employed to carry out morphology characterization and in situ chemical analysis

but higher standards of experimental methods are necessary because of the diversity of biomaterials

the complexity of newly created nanostructures and their functionalities tightly connected with living cells/organs.Therefore

the strategies to develop advanced EM hardware and software required by these specialties of bio nanomaterials are systematically explained.Besides

the development directions for EM in field of bio nanomaterials research such as large size mosaic image with high resolution

three dimensional modeling of tiny and complicate structures

real time and in situ characterization and analysis during experiments are prospected.