Magnetic labeling detection technology based on magnetic nanomaterials has the advantages of high sensitivity，wide linear range and easy operation，while the traditional labeling detection technology based on optical label usually could achieve qualitative detection or semi quantitative detection only.Most biological samples have low magnetic signal，leading to low background interference.Therefore，the magnetic labeling detection technology has shown higher sensitivity than optical labeling detection technology.It could not only carry out quantitative detection，but also reduce the pretreatment time.Point of care testing(POCT) could be performed at or near the sites of patient care，which is the trend of medical diagnosis.Magnetic labeling detection technology has an application potential in POCT，especially in biomedical use，such as detecting protein，nucleic acid，cell，pathogens and biological tissue.The latest research progress of magnetic nanoparticles in POCT is focused in this paper，and their applications in detecting protein，nucleic acid and several kinds of pathogens are discussed.Finally，the development direction and application prospect of POCT technology based on magnetic nanoparticles are outlined.