1.深圳职业技术大学 食品药品学院,广东 深圳 518055
2.大连海洋大学 食品科学与工程学院, 辽宁 大连 116023
3.杭州辛选网络科技有限公司,浙江 杭州 310051
岳振峰,博士,研究员,研究方向:食品质量与安全,E-mail:yuezhenfeng@163.com
收稿:2025-04-26,
修回:2025-07-08,
录用:2025-07-09,
纸质出版:2025-11-15
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周海鹏,岳振峰,蒋丹,魏思阳.基于非靶向代谢组学技术的泰国茉莉香米鉴别技术研究[J].分析测试学报,2025,44(11):2304-2312.
ZHOU Hai-peng,YUE Zhen-feng,JIANG Dan,WEI Si-yang.Study on Authentication Technology for Thai Hom Mali Rice Based on Non-targeted Metabolomics Technology[J].Journal of Instrumental Analysis,2025,44(11):2304-2312.
周海鹏,岳振峰,蒋丹,魏思阳.基于非靶向代谢组学技术的泰国茉莉香米鉴别技术研究[J].分析测试学报,2025,44(11):2304-2312. DOI: 10.12452/j.fxcsxb.250426326.
ZHOU Hai-peng,YUE Zhen-feng,JIANG Dan,WEI Si-yang.Study on Authentication Technology for Thai Hom Mali Rice Based on Non-targeted Metabolomics Technology[J].Journal of Instrumental Analysis,2025,44(11):2304-2312. DOI: 10.12452/j.fxcsxb.250426326.
该研究旨在通过非靶向代谢组学技术建立茉莉香米与非香籼米的鉴别模型,以应对全球香米市场上日益严重的掺假问题。利用液相色谱-高分辨率质谱技术对泰国茉莉香米与我国非香籼米进行了广泛的代谢物谱解析,通过Mann-Whitney U检验、热图对比分析和主成分分析(PCA)筛选出4-羟基-3,5-二甲氧基肉桂酸、咖啡酸、
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-酪氨酸、吲哚和4-羟基喹啉等关键差异化合物,并构建了基于随机森林算法的香米真伪鉴别模型。该模型在建模组和测试组的鉴别准确率达到100%,实际样本的总体鉴别准确率达到95%。该研究为泰国茉莉香米的品质鉴定、市场监管以及消费者权益保障提供了重要的技术支撑。
This study aims to address the growing problem of adulteration in the global fragrant rice market by establishing a non-targeted metabolomic model for distinguishing jasmine rice from non-fragrant rice. Liquid chromatography-high-resolution mass spectrometry was used to analyze the metabolomic profiles of Thai Hom Mali Rice and Chinese non-fragrant indica rice. Key differential metabolites such as 4-hydroxy-3,5-dimethoxy cinnamic acid,caffeic acid,
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-tyrosine,indole,and 4-hydroxyquinoline were identified through the Mann-Whitney U test,heat map a
nalysis,and principal component analysis(PCA). A discrimination model based on the random forest algorithm was developed,achieving 100% accuracy in both the modeling and testing groups,and 95% accuracy in real samples. This research offers a valuable technical solution for ensuring fragrant rice quality,regulating the market,and protecting consumer rights.
Zhao R K . China Business Update (赵染可 . 中国经贸) , 2020 ,( 13 ): 114 - 116 .
Wu Y J , Zhang Z M , Wang B , He X Y . Grain Oil Food Sci . Technol. (吴亚君,张振民,王斌,何小燕. 粮油食品科技), 2011 , 19 ( 5 ): 39 - 42 .
Zhao X , Wang B T , Zhang S X , Jin B H , Zhao Q H , Liang S W , Yan Z , Chen B , Xie L Q . J . Changzhou Univ. : Nat. Sci. Ed . (赵旭,王丙涛,张思娴,靳保辉,赵琼晖,梁淑雯,颜治,陈波,谢丽琪. 常州大学学报:自然科学版), 2018 , 30 ( 4 ): 36 - 40 .
Zhao X , Wang B T , Zhang S X , Jin B H , Zhao Q H , Liang S W , Yan Z , Chen B , Xie L Q . J . Chin. Cereals Oils Assoc . (赵旭,王丙涛,张思娴,靳保辉,赵琼晖,梁淑雯,颜治,陈波,谢丽琪. 中国粮油学报), 2018 , 33 ( 7 ): 118 - 122 .
Su H , Si L L , Qin F , Chen Z C , Shi J M , Li B , Luo Z F , Wang W L . J . Food Saf. Qual . (苏华,司露露,秦富,陈展册,石佳明,李冰,罗兆飞,汪文龙. 食品安全质量检测学报), 2022 , 13 ( 6 ): 1983 - 1988 .
Li Y L , Zheng Y J , Tang L , Su Z Y , Xiong C . Spectrosc. Spectral Anal. (黎永乐,郑彦婕,汤璐,苏志义,熊岑. 光谱学与光谱分析), 2016 , 36 ( 3 ): 834 - 837 .
Xia Q L , Kang S M , Jin Z , Xu J J , Cao L L , Pang M . J . Food Saf. Qual . (夏巧丽,康圣美,金卓,许静静,操丽丽,庞敏. 食品安全质量检测学报), 2023 , 14 ( 12 ): 62 - 70 .
Tang X R . Proceedings of the 20th Annual Conference of China Crop Society(唐湘如 . 第二十届中国作物学会学术年会论文集) , 2023 .
Bi J , Zhang S S , Li M , Zhang J N , Chen D W . J. Instrum. Anal. (毕军,章沙沙,李猛,张嘉宁,陈达炜. 分析测试学报), 2025 , 44 ( 4 ): 602 - 610 .
Bao G , Ashraf U , Li L , Qiao J , Wang C , Zheng Y . Plants , 2024 , 13 : 49 .
Bao T N , Yang Y , Wang B H . Mod . Food Sci. Technol. (包塔娜,杨洋,王柏辉. 现代食品), 2022 , 28 ( 24 ): 202 - 205 .
Liu J , Zhang Y G , Wang P K , Zhao T , Zhao L W , Wu L M . Apic. China (刘进,张永贵,汪平凯,赵彤,赵柳微,吴黎明. 中国蜂业), 2022 , 73 ( 4 ): 60 - 64 .
Li M , Gong Z G , Yang L , Manatbai B , Lü S , Zhu J Y , Fang F , Jia W . J. Instrum. Anal . 李密,巩志国,杨丽,巴哈提古丽·马那提拜,吕帅,朱姬颖,房芳,贾玮. 分析测试学报), 2024 , 43 ( 9 ): 1417 - 1424 .
Yao H Y . Cereal Feed Ind. (姚惠源 . 粮食与饲料工业) , 1997 ,( 9 ): 3 - 4,7 .
Hinge V , Patil H , Nadaf A . Appl. Biochem. Biotechnol. , 2015 . DOI: 10.1007/s12010-015-1898-2 http://dx.doi.org/10.1007/s12010-015-1898-2 .
Kongpun A , Pusadee T , Jaksomsak P , Chinachanta K , Tuiwong P , Chan-In P , Konsaeng S , Pathom-Aree W , Utasee S , Wangkaew B , Prom-U-Thai C . Rice Sci. , 2024 , 31 ( 2 ): 142 - 158 .
Routray W , Rayaguru K . Food Rev . Int. , 2017 . DOI: 10.1080/87559129.2017.1347672 http://dx.doi.org/10.1080/87559129.2017.1347672 .
Xie W J , Ashraf U , Zhong D T , Lin R B , Xian P Q , Zhao T , Feng H Y , Wang S L , Duan M Y , Tang X R , Mo Z W . Food Sci. Nutr. , 2019 , 7 : 3784 - 3796 .
Gianluca T , Concetta C , Fabrizio C , Maria M , Antonella V . J. Sci. Food Agric. , 2020 , 100 ( 3 ): 1328 - 1335 .
Issaraporn J , Wannee J . J. Nutr . Sci. Vitaminol. , 2019 , 65 ( Suppl. ): S231 - S234 .
Liu Y . Study on Quality Analysis and Characteristic Flavor Components of Thai Rice and Wuchang Rice . Shenyang : Shenyang Agricultural University (刘瑶. 泰国香米和五常大米品质分析和特征性风味成分的研究. 沈阳:沈阳农业大学), 2018 .
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