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北京市食品检验研究院(北京市食品安全监控和风险评估中心) 国家市场监管重点实验室(食品安全重大综合保障 关键技术),北京 100094
宋丽萍,博士,正高级工程师,研究方向:食品安全检测,E-mail:slp_0705@sina.com
收稿日期:2025-01-22,
修回日期:2025-03-06,
纸质出版日期:2025-09-15
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李庆尧,索一平,王青龙,曹悦,汤娱涵,邢超,宋丽萍.应用流式细胞仪评估北京市售冷藏预调理牛排的卫生情况[J].分析测试学报,2025,44(09):1764-1768.
LI Qing-yao,SUO Yi-ping,WANG Qing-long,CAO Yue,TANG Yu-han,XING Chao,SONG Li-ping.Assessing the Hygiene of Retail Refrigerated Pre-marinated Beef Steaks in Beijing via Flow Cytometry[J].Journal of Instrumental Analysis,2025,44(09):1764-1768.
李庆尧,索一平,王青龙,曹悦,汤娱涵,邢超,宋丽萍.应用流式细胞仪评估北京市售冷藏预调理牛排的卫生情况[J].分析测试学报,2025,44(09):1764-1768. DOI: 10.12452/j.fxcsxb.25012255.
LI Qing-yao,SUO Yi-ping,WANG Qing-long,CAO Yue,TANG Yu-han,XING Chao,SONG Li-ping.Assessing the Hygiene of Retail Refrigerated Pre-marinated Beef Steaks in Beijing via Flow Cytometry[J].Journal of Instrumental Analysis,2025,44(09):1764-1768. DOI: 10.12452/j.fxcsxb.25012255.
为了快速、精准评估冷藏预调理牛排的卫生状况,该文建立了一种基于流式细胞仪的菌落总数检测方法。T检验结果显示,流式细胞仪检测结果与国家标准方法间无显著性差异(
p
>
0.05),皮尔逊相关性分析发现两种方法存在显著相关性(
p
<
0.001),说明可以应用流式细胞术替代国家标准对冷藏预调理牛排中的菌落总数进行分析。卡方检验结果显示,不同超市销售的牛排在卫生状况上无显著性差异,表明终端销售温度控制并非影响牛排卫生状况的主要因素,最后通过宏基因组溯源确定牛排卫生状况与生产环境的卫生控制密切相关。
In order to assess the hygiene of retail refrigerated pre-marinated beef steaks rapidly and accurately,this study developed a colony count detection method based on flow cytometry. The T-test results showed no significant difference between the flow cytometry results and the national standard method(
p
<
0.05),and Pearson’s correlation analysis demonstrated a significant relationship between the two meth
ods(
p
<
0.001). This indicates that flow cytometry can be used to replace the GB 4789.2 national standard for analyzing colony counts in refrigerated pre-marinated beef steaks. Further,Chi-square analysis revealed no significant differences in hygiene among beef steaks sold in different supermarkets,suggesting that temperature control at the retail level is not the main factor affecting beef steak hygiene. Further metagenomic tracing confirmed that the hygiene of beef steaks is closely related to the hygiene control of the production environment.
RegulationEC) No 2073 / 2005 of the European Parliament and of the Council on Microbiological Criteria for Foodstuffs . http://data.europa.eu/eli/reg/2005/2073/oj http://data.europa.eu/eli/reg/2005/2073/oj .
AS 4696 :2007. Hygienic Production of Meat for Human Consumption . Standard Association of Australia .
GB 4789.2 . National Food Safety Standard-Microbiological Examination of Foods-Total Plate Count . National Standards of the People’s Republic of China(食品安全国家标准 食品微生物学检验 菌落总数测定.中华人民共和国国家标准) .
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Buzatu D A , Moskal T J , Williams A J , Cooper W M , Mattes W B , Wilkes J G . Plos One , 2014 , 9 ( 4 ): e94254 .
Subires A , Yuste J , Capellas M . Int . J. Food Microbiol. ,2014,( 168 / 169 ): 47 - 56 .
He S B , Hong X Y , Zhang M M , Wu L , Yan X M . Anal. Chem. , 2020 , 92 ( 3 ): 2393 - 2400 .
Zhao X M , Wang P , Qu T M , Liang L J , Ji Q L , Chen Y . Qual. Saf. Inspect. Test. (赵晓美,王聘,曲天铭,梁丽娇,姬庆龙,陈颖. 质量安全与检验检测), 2023 , 33 ( 1 ): 7 - 15 .
Williams A , Gaoh S D , Savenka A , Paredes A , Alusta P , Ahh Y B , Buzatu D A . Front. Microbiol. , 2024 , 15 : 1342478 .
Ou L J , Li L , Yang H , Yang F X , Xu E X , Zhou Q Y . Packag. Eng. (欧丽娟,李立,杨辉,杨福馨,徐尔萱,周沁怡. 包装工程), 2015 , 36 ( 5 ): 649 - 654 .
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