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山东省产品质量检验研究院,山东 济南 250102
贺祥珂,研究员,研究方向:色谱质谱分析、健康风险评估,E-mail:hexiangke@sdqi.com.cn
高翠玲,博士,研究员,研究方向:消费品化学安全检测与风险评估,E-mail:gaocuiling@sdqi.com.cn
收稿日期:2024-11-14,
修回日期:2024-12-11,
录用日期:2025-01-21,
纸质出版日期:2025-07-15
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李桂晓,尹畅,王月,范红梅,彭晓辉,贺祥珂,高翠玲.高效阴离子交换色谱-积分脉冲安培法测定化妆品中甘油葡糖苷[J].分析测试学报,2025,44(07):1439-1442.
LI Gui-xiao,YIN Chang,WANG Yue,FAN Hong-mei,PENG Xiao-hui,HE Xiang-ke,GAO Cui-ling.Determination of Glyceryl Glucoside in Cosmetics by High Performance Anion Exchange Chromatography-Integrated Pulse Amperometry[J].Journal of Instrumental Analysis,2025,44(07):1439-1442.
李桂晓,尹畅,王月,范红梅,彭晓辉,贺祥珂,高翠玲.高效阴离子交换色谱-积分脉冲安培法测定化妆品中甘油葡糖苷[J].分析测试学报,2025,44(07):1439-1442. DOI: 10.12452/j.fxcsxb.241114526.
LI Gui-xiao,YIN Chang,WANG Yue,FAN Hong-mei,PENG Xiao-hui,HE Xiang-ke,GAO Cui-ling.Determination of Glyceryl Glucoside in Cosmetics by High Performance Anion Exchange Chromatography-Integrated Pulse Amperometry[J].Journal of Instrumental Analysis,2025,44(07):1439-1442. DOI: 10.12452/j.fxcsxb.241114526.
建立了一种测定化妆品中甘油葡糖苷的方法。以10%甲醇水为萃取溶剂,超声提取,涡旋振荡取上清液经滤膜过滤后上机测试。采用Hamilton RCX-30阴离子交换色谱柱,脉冲安培检测器,金工作电极,流速1 mL/min,离子色谱仪测定,外标法定量,对提取溶剂、时间、基质效应进行了分析研究。结果表明,甘油葡糖苷在0.1~5.0 mg/L范围内线性良好,在3种加标水平下的回收率为96.0%~108%,相对标准偏差为1.2%~5.0%,相关系数为0.999 7,检出限为0.2 mg/kg。该方法操作简单高效,具备较好的重复性和稳定性,适用于化妆品中甘油葡糖苷含量的准确测定。
A method for determination of glyceryl glucoside in cosmetics was established in this paper. The sample is extracted with 10% methanol water by ultrasonic extraction and vortex shaking,and the supernatant was filtered through a filter membrane before being tested on the instrument. The system used was a Hamilton RCX-30 anion exchange column,a pulse amperometric detector,a gold working electrode,a flow rate of 1 mL/min,ion chromatography measurement,and external standard quantification. The extraction solvent,extraction time,matrix effect were analyzed and studied. The results showed that glyceryl glucoside had good linearity within the concentration range of 0.1-5.0 mg/L,with recoveries of 96.0%-108% and relative standard deviations of 1.2%-5.0%,and correlation coefficient of 0.999 7. The detection limit is 0.2 mg/kg. The method is simple,efficient,and has good repeatability and stability,making it suitable for the accurate determination of glyceryl glucoside in cosmetics.
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