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Evaluation of the Assembly Algorithms for De Novo Sequencing of Antibodies and Their Application in Analyzing Pan-coronavirus Neutralizing Antibodies
Scientific Papers | 更新时间：2026-03-11

    • Evaluation of the Assembly Algorithms for De Novo Sequencing of Antibodies and Their Application in Analyzing Pan-coronavirus Neutralizing Antibodies

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    • “This article introduces its research progress in the field of antibody development. Relevant experts used integrated multi enzyme gradient enzymatic hydrolysis and mass spectrometry strategies to perform de novo sequencing on three humanized pan coronavirus neutralizing antibodies. By comparing the performance of the three assembly algorithms, it was found that the Fusion algorithm performed excellently in light and heavy chain coverage and accuracy, providing a reliable sequence analysis basis for the rational design and development of pan coronavirus neutralizing antibodies.”
    • Journal of Instrumental Analysis   Vol. 45, Issue 3, Pages: 551-562(2026)

    • DOI：10.12452/j.fxcsxb.25100901    

      CLC： O657.63;R915

    • Received：09 October 2025，

      Revised：2025-11-21，

      Accepted：28 November 2025，

      Online First：09 February 2026，

      Published：15 March 2026

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  • XIONG Yue-ting,XIAO Jin,JIANG Wen-bin,LIU Ying,YUAN Quan.Evaluation of the Assembly Algorithms for De Novo Sequencing of Antibodies and Their Application in Analyzing Pan-coronavirus Neutralizing Antibodies[J].Journal of Instrumental Analysis,2026,45(03):551-562. DOI： 10.12452/j.fxcsxb.25100901.

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