FAN Shuai-qiang,YOU Qing-xiang,ZENG-yuan ,YANG Bo,MA Ming-yuan,ZHANG Da-wei,LI Zhen-qing.Research on Online Analysis of Protein by Gel Electrophoresis[J].Journal of Instrumental Analysis,2025,44(10):2204-2208.
FAN Shuai-qiang,YOU Qing-xiang,ZENG-yuan ,YANG Bo,MA Ming-yuan,ZHANG Da-wei,LI Zhen-qing.Research on Online Analysis of Protein by Gel Electrophoresis[J].Journal of Instrumental Analysis,2025,44(10):2204-2208. DOI: 10.12452/j.fxcsxb.25011739.
Research on Online Analysis of Protein by Gel Electrophoresis
Based on the self-built protein electrophoresis instrument,this paper proposes a method that can realize the SDS-PAGE separation of proteins,and online analysis of its molecular weight and concentration. Through electrophoresis of 10.5-107 kDa protein Marker,and the relationship between its gray value and protein molecular concentration is analyzed by analysis of its gel electrophoresis images. Results showed that:the device can achieve rapid separation of protein samples with a molecular weight of less than 107 kDa within 30 min,and the fluorescence intensity of each protein band reaches its peak when irradiated with ultraviolet light for 5 min;Within the range of 10.5-107 kDa,the migration distance is directly proportional to the molecular weight,with a correlation coefficient of up to 0.93;For protein molecules above 20.1 kDa,the fluorescence intensity shows a linear relationship in the concentration range of 0.72-3.96 μg/μL with with correlation coefficients more than 0.988;Using the developed protein electrophoresis instrument analyzing the protein components in tears,and immunoglobulin A,lactoferrin,serum protein,lipid transport protein,and lysozyme were successfully separated from tears. Compared with the traditional protein gel electrophoresis methods,the developed self-built protein electrophoresis instrument could shorten the protein electrophoresis time from over 1.5 h to within 30 min. This study was important for the development of rapid protein molecule detection equipment.
关键词
Keywords
references
Yan J Z , Yan Z X . J. Phys. Sci. Appl. , 2019 , 9 ( 1 ): 34 - 42 .
Obeagu E I , Ochei K C , Nwachukwu B N , Nchuma B O . Scholars J. Appl. Med. Sci. , 2015 , 3 : 2244 - 2252 .
Wang J Y , Wu X , Yao Z P . J. Instrum Anal . (王健莹,吴茜,姚钟平. 分析测试学报), 2020 , 39 ( 1 ): 44 - 49 .
Hajba L , Jeong S , Chung D S , Guttman A . TrAC,Trends Anal. Chem. , 2023 , 162 : 117024 .
Sugiyama Y , Uezato Y . J. Proteom. , 2022 , 255 : 104485 .
Lewis A , Cranny A , Harris N , Green N G , Wharton J A , Wood R , Stokes K R . Meas. Sci. Technol. , 2013 , 24 : 042001 .
Li J , Peng B , Li X , Luo D . Colloids Surf. A , 2024 , 681 : 132726 .
Otzen D E , Pedersen J N , Rasmussen H Ø , Pedersen J S . Adv. Colloid Interface Sci. , 2022 , 308 : 102754 .
Shi Y , Mowery R A , Ashley J , Hentz M , Ramirez A J , Bilgicer B , Slunt‐Brown H , Borchelt D R , Shaw B F . Protein Sci. , 2012 , 21 : 1197 - 1209 .
Pergande M R , Cologna S M . Proteomes , 2017 , 5 : 4 .
Kubáň P , Kubáň P . TrAC,Trends Anal. Chem. , 2023 , 159 : 116941 .
Rajeswari K , Anand M T , Mahendran R . ACS Food Sci. Technol. , 2024 , 4 : 1979 - 1996 .
Xue J , Zhang Q , Cao J , Tian Y , Zha G , Liu X , Liu W , Wang Y , Gui D , Cao C . Anal. Chem. , 2021 , 94 : 2007 - 2015 .
Zhang Y , Zhang Y , Shi X J , Li J X , Wang L H , Xie C E , Wang Y L . Front. Oncol. , 2022 , 12 : 836333 .
Application of Fluorescent Probes in Protein Phosphorylation and Glycosylation Detection
Research Progress on Nucleic Acid-mediated Protein Covalent Modification
Study on Separation of Proteins by Pulsed Field Capillary Electrophoresis
Related Author
LI Zhen-qing
YOU QING-xiang
YANG Xin-di
QING Guang-yan
SHAO Juan
LI Jun-rong
CHANG Yong-xin
YANG Huang-hao
Related Institution
Changzhou Institute of Technology
State Key Laboratory of Fine Chemicals, Dalian University of Technology
CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences
MOE Key Laboratory for Analytical Science of Food Safety and Biology,State Key Laboratory of Photocatalysis on Energy and Environment,College of Chemistry,Fuzhou University
Photonics Advanced Research Center, Osaka University, Osaka 565-