JIN Shan-shan,CHANG Jing,WU Shi-hao,DONG Lin-pei,XIN Guo-bin,WANG Rui-hua.Simultaneous Determination of Catecholamines and Their Metabolites in Blood by UPLC-MS/MS Method[J].Journal of Instrumental Analysis,2025,44(07):1378-1384.
JIN Shan-shan,CHANG Jing,WU Shi-hao,DONG Lin-pei,XIN Guo-bin,WANG Rui-hua.Simultaneous Determination of Catecholamines and Their Metabolites in Blood by UPLC-MS/MS Method[J].Journal of Instrumental Analysis,2025,44(07):1378-1384. DOI: 10.12452/j.fxcsxb.25011426.
Simultaneous Determination of Catecholamines and Their Metabolites in Blood by UPLC-MS/MS Method
A method based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was established for the simultaneous determination of catecholamines(CAs) and their three metabolites in blood. This method requires only 100 µL of blood sample,which is purified using an ultrafiltration centrifuge tube and extracted by solid-phase extraction. Separation is achieved using an HSS PFP column,with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution. The samples were scanned by electrospray positive ion multiple reaction monitoring(MRM) mode and quantified by internal standard method. The results showed that the linearities of CAs and their metabolites were good in the concentration range of 0.5-50 ng/mL(
r
²
>
0.99). The limits of detection and quantification were 0.2 ng/mL and 0.5 ng/mL,respectively. The recoveries of six target analytes at spiked levels of 1,10 and 50 ng/mL ranged from 68.9%-101%,with matrix effects ranging from 86.2%-102%,and the relative standard deviations(RSDs) from 2.3%-10%. The established method can provide effective technical means for clinical diagnosis and the detection and identification of epinephrine poisoning cases.
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