LI Shu-fang,LIU Wen-bin,FENG Shu-hui,YANG Ya-qin,HU Yong-jian,HAO Xue-fei,CHENG Ying-ying,YU Yong-jie.Differential Analysis of Different Varieties of Lentinula Edodes Based on Untargeted Metabolomics[J].Journal of Instrumental Analysis,2025,44(10):2143-2152.
LI Shu-fang,LIU Wen-bin,FENG Shu-hui,YANG Ya-qin,HU Yong-jian,HAO Xue-fei,CHENG Ying-ying,YU Yong-jie.Differential Analysis of Different Varieties of Lentinula Edodes Based on Untargeted Metabolomics[J].Journal of Instrumental Analysis,2025,44(10):2143-2152. DOI: 10.12452/j.fxcsxb.24122331.
Differential Analysis of Different Varieties of Lentinula Edodes Based on Untargeted Metabolomics
The chemical components of fruiting body from different varieties of
Lentinula edodes
are directly associated to their sensory quality,nutritional efficacy and even processing performance. In the present work,an untargeted metabolomics method was developed to analyze the nonvolatility compounds of different varieties of
Lentinula edodes
(9608,Qihe No.9,Yusen No.3). The raw data of
Lentinula edodes
samples were collected by ultrahigh performance liquid chromatography-high resolution mass spectrometry(UHPLC-HRMS) under data-dependent acquisition mode. The acquired UHPLC-HRMS data files were directly imported into a recently proposed chemometric automatic data analysis software AntDAS-DDA to accomplish the metabolomics analysis. The software integrates UHPLC-HRMS data reading and resolution,as well as compound identification,multi-group sample batch processing,and chemometric modeling into one platform. After the comprehensive analysis of results under positive and negative ion modes,66 differential metabolites were identified and 14 compounds were verified by standards. Among them,6 compounds could be identified and validated in both modes,including glutamic acid,phenylalanine,adenine,xanthine,guanosine,and riboflavin. Finally,hierarchical clustering analysis and principal component analysis were used to realize the discr
iminant analysis of
Lentinula edodes
samples from various varieties based on the metabolite identification. The studies indicated that the metabolites of different varieties of
Lentinula edodes
had unique qualities of their own,including high levels of bitter amino acids and nucleotides in 9608,vitamin and umami amino acids in Yusen No.3,as well as purine and arginine in Qihe No.9,which would provide guidance for the varieties breeding,quality evaluation and resource utilization in the future research of
Lentinula edodes
.
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