HE Bin,ZHOU Wei-hao,GAO Xian-hui,ZHANG Hai,CHEN Jing-yue,WANG Yu-tong,YANG De-zhi,YANG Ya-ling.Detection of Free Amino Acids in Tobacco Leaves Based on Zn/NA-CDs Fluorescent Probe[J].Journal of Instrumental Analysis,2025,44(10):2177-2183.
HE Bin,ZHOU Wei-hao,GAO Xian-hui,ZHANG Hai,CHEN Jing-yue,WANG Yu-tong,YANG De-zhi,YANG Ya-ling.Detection of Free Amino Acids in Tobacco Leaves Based on Zn/NA-CDs Fluorescent Probe[J].Journal of Instrumental Analysis,2025,44(10):2177-2183. DOI: 10.12452/j.fxcsxb.24120907.
Detection of Free Amino Acids in Tobacco Leaves Based on Zn/NA-CDs Fluorescent Probe
In this study,a new type of zinc-doped carbon point(Zn/NA-CDs) was synthesized by hydrothermal method using epinephrine and zinc chloride as precursor. The optical properties of Zn/NA-CDs were studied by UV-Vis spectrophotometry and fluorescence spectrophotometry. The structural characteristics of Zn/NA-CDs were studied by X-ray photoelectron spectroscopy(XPS),transmission electron microscope(TEM),Fourier transform infrared spectroscopy(FT-IR),and X-ray diffraction(XRD). Zn/NA-CDs showed a relatively high photoluminescent quantum yield of about 43.28%. In this study,amino acids could react with phthalic aldehyde(OPA) to produce schiff base products,which can produce fluorescence sensitization on Zn/NA-CDs. Under the optimal analysis conditions,with glycine as the model molecule,the fluorescence intensity of the system was linear with the concentration of glycine in the range of 2.5-250 mg/L,and the detection limit was 0.6 mg/L. The method can be used for the determination of amino acids in actual tobacco samples,and satisfactory test results were obtained.
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