YAO Meng-di,GAO Yu,SUN Shu-yang,LIN Li,ZHANG Nuo-xuan,WANG Ping,WANG Cheng-ke.HRP-loaded Nanocomposite Enzyme for Colorimetric Determination of Hypoxanthine in Oysters[J].Journal of Instrumental Analysis,2025,44(10):2191-2197.
YAO Meng-di,GAO Yu,SUN Shu-yang,LIN Li,ZHANG Nuo-xuan,WANG Ping,WANG Cheng-ke.HRP-loaded Nanocomposite Enzyme for Colorimetric Determination of Hypoxanthine in Oysters[J].Journal of Instrumental Analysis,2025,44(10):2191-2197. DOI: 10.12452/j.fxcsxb.241203571.
HRP-loaded Nanocomposite Enzyme for Colorimetric Determination of Hypoxanthine in Oysters
nanocomposite materials,which improved the loading efficiency and stability of horseradish peroxidase(HRP),and used it for the sensitive colorimetric detection of hypoxanthine(HX) in oysters. As the product of the reaction between HX and xanthine
oxidase(XOD),the H
2
O
2
intermediate can oxidize the Amplex red(AR),and form a red product under the catalysis of the Fe
3
O
4
@ZIF-8@HRP@SiO
2
nanoenzyme complex,thus enabling the quantitative analysis of HX. The material's morphology,elemental composition,and surface functional groups were characterized using transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),and particle size analysis. Under the optimal conditions,the detection range for HX is 0.005-50 μmol/L,with a limit of detection of 0.001 27 μmol/L. Compared with the traditional methods such as capillary electrophoresis and electrochemical methods,it has a wider linear range and higher detection sensitivity. This method was also used for detecting the HX content changes in oysters that were stored continuously for 96 hours at 4 ℃ and 30 ℃,further verifying its feasibility for detecting actual samples.
关键词
Keywords
references
Bonilla A C , Sveinsdottir K , Martinsdottir E . Food Control , 2007 , 18 ( 4 ): 352 - 358 .
Albelda J A V , Uzunoglu A , Santos G N C , Stanciu L A . Biosens. Bioelectron. , 2017 , 89 : 518 - 524 .
Chen J M , Lu Y , Yan F , Wu Y Z , Huang D , Weng Z Q . Food Chem. , 2020 , 310 : 125922 .
Chen Z T , Lin Y , Ma X M , Guo L H , Qiu B , Chen G N , Lin Z Y . Sens. Actuators B , 2017 , 252 : 201 - 208 .
Raj M , Moon J M , Goyal R N , Park D S , Shim Y B . Biosens. Bioelectron. , 2019 , 126 : 758 - 766 .
Li D P , Qin N , Zhang L T , Li Q , Prinyawiwatkul W , Luo Y K . Int. J. Refrig. , 2019 , 98 : 294 - 301 .
Yin X H , Yang X J , Luo D , Zhang Y L , Wang H B , Yang W R , Pang P F . J. Instrum. Anal. (尹学虎,杨新杰,罗丹,张艳丽,王红斌,杨文荣,庞鹏飞. 分析测试学报), 2023 , 42 ( 3 ): 315 - 322 .
Zhang Y H , Liu X T , Xu G F , Wang W J , Wang X M , Li Q , Xu R , Du Z Y . Shandong Chem. Ind. (张义豪,刘笑彤,徐国峰,王文君,王晓敏,李群,许瑞,杜中玉. 山东化工), 2024 , 53 ( 1 ): 110 - 112 .
Li J M , Yan F , Huang B B , Zhang M Y , Wu X D , Liu Y H , Ruan R , Zheng H L . Appl. Biochem. Biotechnol. , 2024 , 196 ( 8 ): 5403 - 5418 .
Liu D M , Chen J , Shi Y P . Trends Anal. Chem. , 2018 , 102 : 332 - 342 .
Taheri-Kafrani A , Kharazmi S , Nasrollahzadeh M , Soozanipour A , Ejeian F , Mansouri-Tehrani H , Razmjou A , Yek S , Varma R . Crit. Rev. Food Sci. Nutr. , 2021 , 61 ( 19 ): 3160 - 3196 .
Rodrigues R C , Berenguer-Murcia Á , Carballares D , Morellon-Sterling R , Fernandez-Lafuente R . Biotechnol. Adv. , 2021 , 52 : 107821 .
Chu G B , Li W Y , Han X X , Sun H H , Han Y , Zhi G Y , Zhang D H . Small , 2023 , 19 ( 26 ): 2301413 .
Jia H , Yue X , Hou Y , Huang F , Cao C Y , Jia F F , Liu G H , Zheng X B , Liu Y T , Jiang Y J . Front. Chem. Sci. Eng. , 2024 , 18 ( 10 ): 114 .
He G Y , Liu H R , Yang C L , Hu K S , Zhai X C , Fang B B , Liu K X , Zulekha , Li D L . Biochem. Eng. J. , 2024 , 204 : 109207 .
Liu Y Y , Cheng A D , Wu Y Z , Han S , Peng F . Mod. Food Sci. Technol. (刘英语,程爱迪,吴酉芝,韩帅,彭飞,现代食品科技), 2022 , 38 ( 11 ): 358 - 366 .
Iriarte-Mesa C , Pretzler M , Von Baeckmann C , Kahlig H , Krachler R , Rompel A , Kleitz F . J. Colloid Interface Sci. , 2023 , 646 : 413 - 425 .
Wang Y P , Li Z H , Fu W L , Sun Y M , Dai Y Q . Adv. Fiber Mater. , 2022 , 4 ( 5 ): 1278 - 1289 .
Parizadeh L , Tourbiez D , Garcia C , Haffner P , Dégremont L , Le Roux F , Travers M . Environ. Microbiol. , 2018 , 20 ( 12 ): 4343 - 4355 .
Zhao Z X , Yang X , Wei J , Chen X M . Food Sci. (赵子轩,杨雪,魏洁,陈晓梅. 食品科学), 2021 : 246 - 251 .
Guo C , You S H , Li C M , Chen T T , Wang X D . Front. Microbiol. , 2021 , 12 : 791227 .
Avan A N , Karakaş Ö , Demirci-Çekiç S , Apak R . Enzyme Microb. Technol. , 2023 , 162 : 110137 .