The sensitive and rapid detection of clenbuterol ractopamine(RAC) is of great significance for food safety and human health. However,the traditional RAC analysis methods often involve complex instruments and professional operators,and it is difficult to achieve portable field detection. Using the cation exchange reaction(CER) between cadmium telluride quantum dots(CdTe QDs) and silver ion(Ag
+
),and the specific recognition properties of aptamer(Apt),combined with enzyme-free hairpin assembly(CHA) of signal amplification,a low-cost,high-sensitivity field quantitative detection method for ractopamine was proposed. The detection limit was a
s low as 2.3 pmol·L
-1
,significantly lower than the maximum residue limit(MRL) recommended by the Codex Alimentarius Commission. The key of this method was that the aptamer recognizes RAC to drive cytosine-Ag
+
-cytosine structure in hairpin DNA to release Ag
+
,while effectively promoting the occurrence of multiple CERs through CHA and continuously inducing fluorescence quenching of CdTe QDs,which provided a basis for highly sensitive field quantitative analysis of RAC. The practical feasibility of the method was verified,including its stability,repeatability,and performance over non-CHA systems. This method can not only distinguish different contents of RAC with open eyes,but also has universality. It can be used to analyze other target objects only by modifying the identification region in the system. This study is conducive to the advancement of food safety and environmental health,and provides a highly efficient tool for quantitative analysis of RAC and other potentially similar compounds.
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references
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